Scanning laser confocal microscope image of a collagen-fibrinogen freeze-dried scaffold.
Figure: 3D micro-tomography images, demonstrating the differences in architecture achievable on variation of the thermal profile during freezing.
The relationship between time at equilibrium and pore size appeared to be universal regardless of changes to the slurry, to the mould design, to the set freezing protocol or to the mould design. Inset: the slope of the curve was 1/n, where n = 4.8; fit with a linear regression curve ( p < 0.05, R2 = 0.8). (see publication *)
Comparison of normal mammary tissue architecture (top) with organoid architecture of our in vitro model (bottom) showing immunofluorescence staining for lineage specific markers. Bars = 200 µm (leftmost panel) and 50 µm remainder.